The 4th mCBR workshop on “CRISPR/CAS-based Molecular Diagnostics” was organized by (and at) the Department of Medical Biotechnology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission’s Research Foundation, Puducherry on 3rd and 4th August, 2023. The main goal of the workshop was to provide participants with both a theoretical and hands-on understanding of the CRISPR/Cas system, specifically focusing on utilizing the Cas12a enzyme for molecular diagnostic purposes. The workshop was attended by 22 participants from various institutions. The participants comprised of clinicians, MD students, Ph.D. research scholars, and M.Sc. students.